The purpose of this study was to assess the effects of lipid peroxidation with occupational exposure to different types of nanomaterials (NMs). In this cross-sectional study, urine and exhaled breath condensate (EBC) samples were collected from 80 NM-handling workers [30 workers handling nano-titanium oxide (nano-TiO₂), 28 handling nano-silicon dioxide (nano-SiO₂), 22 handling carbon nanotubes (CNTs)], and 69 controls (office workers) from 2010 to 2012. Urinary 8-isoPGF_2α, 2,3 dinor-8-isoPGF_2α, PGF_2α, and EBC 8-iso PGF_2α were measured as lipid peroxidation biomarkers in 2013. A significant positive correlation was found between 8-isoPGF_2α, 2,3 dinor-8-isoPGF_2α, PGF_2α, and total isoprostane in urine. Furthermore, significant positive correlations were noted between EBC 8-iso PGF_2α and urinary 2,3 dinor-8-isoPGF_2α (Spearman correlation r = 0.173, p = 0.035). Exposure to nano-TiO₂ resulted in significantly higher levels of urinary 8-isoPGF_2α, 2,3 dinor-8-isoPGF_2α and PGF_2α, even after controlling for confounding factors. Moreover, significant associations and exposure intensity–response relationships between EBC 8-iso PGF_2α and NMs were observed in workers, whether handling nano-TiO₂, nano-SiO₂, or CNTs. Among them, the significant trends were identified based on the intensity of risk levels. These results provided evidence that exposure to nano-TiO₂, nano-SiO₂, and CNTs may lead to lipid peroxidation in EBC. For routine biomonitoring purposes, this finding, which came through noninvasive methods, may be useful for workers exposed to NMs. [Link to this article...]

KEYWORDS Carbon nanotubes; metal oxide nanomaterials; 8-isoprostaglandin F2a; biomarkers; LC-MS/MS