Two previously undescribed chromone derivatives, 1 and 2, were isolated from the EtOAc-soluble fraction of the culture broth of Trichoderma sp. 18F0056. Their structures were elucidated on the basis of extensive analyses of spectroscopic data and comparison with literature data. Their antifungal activity was also evaluated. Our results showed that both constituents possessed weak antifungal activity against Fusarium sp. LC8, Neopestalotiopsis sp. BCRC 35002, and Colletotrichum gloeosporioides BCRC 35178. In the past, chromone derivatives were rarely found from the genus Trichoderma. This time, the research on the products of this new species of Trichoderma is of significant significance. Trichoderma natural products deserve further evaluation as anti-fungal agents for biocontrol.

Keywords：Trichoderma sp. 18F0056, Chromone, Antifungal, Secondary metabolites